Coding
MazF

Part:BBa_K4060001:Design

Designed by: Yi Rong Chen   Group: iGEM21_NYCU-Taipei   (2021-09-30)


MazF gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 363
    Illegal NotI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 20
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence confirmed.

Source

The fragment is ligated by 2021_NYCU_Taipei using the iGEM kit.

References